Effects of temperature, pH, and photoperiod on the performance of a freshwater cladoceran Moina micrura culture enriched with Lysinibacillus fusiformis and Bacillus pocheonensis

The freshwater cladoceran Moina micrura has tremendous potential for mass culture as a live feed larviculture. This study aimed to evaluate the efficacy of probiotics Lysinibacillus fusiformis A1 and Bacillus pocheonensis S2 in enhancing population density, growth rate, production M. under different environmental conditions. Four temperatures (15, 20, 25, 30°C), pH levels (4, 6, 8, 10), photoperiods (4L:20D, 6L:18D, 8L:16D, 12L:12D) were set up. daily number individuals incubated each parameter was monitored 12 days determine density rate micrura. Meanwhile, neonates from five females kept respective parameters until they died naturally. Probiotics added individually at 5×104 CFU mL-1 twice during first sixth experiment. enriched with B. 30°C had highest maximum (10 ± 0.2 ind mL-1) produced (132 6.43 ind), whereas treatment 20°C best (0.1863 0.006 d-1). normal photoperiod 12L:12D 0.3 (129 4.58 ind) while incubation 8L:16D (0.2879 0.0007 L. 8 (11 0.8 mL-1), (0.5508 0.04 d-1), 4.36 ind). Results recommend that warmer temperature 30°C, alkaline 10, can be adopted enrichment maximize its productivity aquaculture use.